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Science in Seconds - GFP

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Science in Seconds Know Everything RAVES - GFP Torah Kachur: The new trend in pets is glowing. Glowing kittens, glowing fish and even glowing pigs. Discovery of Green Fluorescent Protein has allowed us to engineer Spot to glow. But GFP is more than just a party trick: it represents one of the more important scientific techniques in the last half century. GFP has helped to study HIV resistance, track cancer spread, watch my stink, and even make mosquito testicles glow, which helps us light males to sterilize them and prevent the spread of malaria, not just because glowing testicles are amazing. GFP is a naturally occurring protein in jellyfish, that fluoresces when they get pissed off. The clone gene was used by Martin Chalfie to invent Gloworms. He had a brilliant idea, as a Nobel brilliant, and figured he could make individual proteins visible, by combining the DNA of the GFP protein and the protein he was studying. He spliced together the two genes, injected it back into C.Elegans, and voilà, the worms glowed! We could suddenly see the invisible: the behaviour of individual proteins in a live organism. GFP works because it can absorb instant light at a particular wavelength in the UV spectrum. Then emit a second wavelength of lower energy, that is now in the visible spectrum: green. A myriad of different GFP variants have been developed that fluoresce literally every color of the raimbow. This has allowed some researchers to create awesome pictures of “Brainbows”, where most brain cells are engineered with all the colors of the GFP, and have allowed to turn on the different genes randomly in the brain. The Braimbow's really cool, but has no real purpose: other, more useful applications of GFP-tagged cells have allowed researchers to determine how cats could be made resistant to FIV. They used GFP to track if a macaque gene with no color was successfully introduced into the cat. Then they tested if there were resistance to FIV. GFP has allowed us to peer into the world of the cell in a way that seems voyeuristic. We can see how cells work and watch, for the first time, what happens in a live cell. Courtesy of Science in Seconds - All rights reserved Only for educational / non profit purposes (Translators are welcome to put their name here)

Video Details

Duration: 2 minutes and 17 seconds
Country: United States
Language: English
License: All rights reserved
Producer: Science in Seconds
Director: Science in Seconds
Views: 588
Posted by: tradottiinitaliano on Jan 30, 2012

The Nobel prize in Chemistry in 2008 was awarded to 3 scientists that developed green fluorescent protein (GFP) as a technique that makes organisms glow. This is more than just some fun body paint and a rave or two, the researchers were able to splice together genes from jellyfish and a normal gene that would allow scientists to watch the behaviour of proteins in living cells.

This gave rise to neon rabbits in art exhibits, fluorescent-snouted pigs and even red-glowing paws in kittens. GFP has revolutionized the way we see into cells and our understanding of the fundamental structure of life.

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