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The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation

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Video Details

Duration: 4 minutes and 10 seconds
Country: China
Producer: Christopher R. Parish
Director: Christopher R. Parish
Views: 286
Posted by: scorpio_liu on Oct 13, 2010

CFSE covalently labels long-lived intracellular molecules with the fluorescent dye, carboxyfluorescein. As such, when a CFSE-labeled cell divides, its progeny have half the amount of fluorescence, which can thereby be used to assess cell division. This article describes the procedures typically used for labeling mouse lymphocytes with CFSE.

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